TRA 1 , a Novel mRNA Highly Expressed in Leukemogenic Mouse Monocytic

Mouse monocytic Mm-A, Mm-P, Mm-S1, and Mm-S2 cells sequence of 201 amino acids from the C-terminal NOR1 was completely identical to that of TRA1, whereas the remaining are sublines of mouse monocytic and immortalized Mm-1 cells derived from spontaneously differentiated, mouse myN-terminal amino acids (33 amino acids) were longer than that (3 amino acids) of TRA1 and the N-terminus of NOR1 eloblastic M1 cells. Although these subline cells retain their monocytic characteristics in vitro, Mm-A and Mm-P cells are protein contained proline-rich sequence. A similarity search against current nucleotide and protein sequence databases highly leukemogenic to syngeneic SL mice and athymic nude mice, whereas Mm-S1 and Mm-S2 cells are not or are only indicated that the NOR1/TRA1 gene(s) is conserved in a wide range of eukaryotes, because apparently homologous genes slightly leukemogenic. To better understand the molecular mechanisms of these levels of leukemogenicity, we investiwere identified in Caenorhabditis elegans and Saccharomyces cerevisiae genomes. Northern blotting using TRA1gated putative leukemogenesis-associated genes or oncogenes involved in the maintenance of growth, especially in specific and NOR1-specific probes indicated that TRA1 mRNA is exclusively expressed in leukemogenic but not in vivo, by means of differential mRNA display. We isolated a fragment clone (15T01) from Mm-P cells. The mRNA probed nonleukemogenic Mm sublines and normal tissues and also indicated that NOR1 mRNA is expressed in normal tissues, with 15T01 was expressed at high levels in leukemogenic Mm-P and Mm-A cells but not in nonleukemogenic Mm-S1 especially in kidney, lung, liver, and bone marrow cells but not in any Mm sublines. After leukemogenic Mm-P cells and Mm-S2 cells. The gene corresponding to 15T01, named TRA1, was isolated from an Mm-P cDNA library. The longest were induced to differentiate into normal macrophages by sodium butyrate, the normal counterpart, NOR1, was exopen reading frame of the TRA1 clone predicts a peptide containing 204 amino acids with a calculated molecular pressed, whereas the TRA1 level decreased. Furthermore, transfection of TRA1 converted nonleukemogenic Mm-S1 weight of 23,049 D. The predicted TRA1 protein is cysteinerich and contains multiple cysteine doublets. A putative norcells into leukemogenic cells. These results indicate that the TRA1 gene is associated at least in part with the leukemomal counterpart gene, named NOR1, was also isolated from a normal mouse kidney cDNA library and sequenced. NOR1 genesis of monocytic Mm sublines. q 1997 by The American Society of Hematology. cDNA predicts a peptide containing 234 amino acids. The